The food chain is the single biggest driver of biodiversity (BD) loss. We create a Life Cycle Assessment (LCA) based toolkit in collaboration with national and global partners for food service operators to help to stop BD- loss. The LCA-based toolkit includes a ranking chart for the 50 most important ingredients based on their BD footprint and provides guidance for food professionals to create biodiversity-friendly meals, model meal recipes for an easy start in food services, and promotional and educational materials that visualize the reduction in BD footprint resulting from changes within food categories. The tool enables food service operators in strategic planning to achieve their sustainability objectives and enhance customers’ confidence in making eco-conscious food choices.